The purposes of this study were to assess seasonal, daily, and gender variations in children’s physical activity (PA). ActiGraph GT3X accelerometers were used to record the three-year-old children’s PA levels for five consecutive days in autumn and winter. Complete data for both seasons were obtained for 47 children. Despite a significant difference in seasonal temperatures (p < .001), differences were only found for weekdays light PA (p = .021). No difference in PA was observed between weekdays and weekend days. Only 20% of the sample had ≥120 minutes light-to-vigorous PA (LMVPA), and 46% of children had ≥60 minutes moderate-to-vigorous PA (MVPA). Boys spent more minutes in LMVPA (p = .001) and MVPA (p = .004) than girls. The current findings indicated that season and day of the week only minimally influence children’s PA levels, whereas gender continues to be a significant factor.