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Prehospital emergency care is a field of work with multiple factors that may cause mental strain. Several of these factors apply to every authority that faces disasters and traumatic incidents in their profession. At worst, these factors may lead to a change of career if appropriate ways to defuse these factors are not available. The purpose of the thesis is to create material to a eDefusing education project on encountering a person in crisis and dealing with mentally stressful and traumatic situations. The aim of the thesis is to increase the authorities’ knowledge of how to deal with crisis and how to process mentally stressful and traumatic situations. The aim is also to maintain good working capacity and reduce burnout. This thesis is practice-based by its character. The first output of the thesis was a script to a simulation case and the second output was a video where a paramedic trained in defusing is interviewed about defusing-sessions. In the future the authorities’ knowledge and experience in encountering patients could be charted on the basis of the education project.
Abstract Mitochondrial fatty acid synthesis (mtFAS) is a highly conserved pathway essential for mitochondrial biogenesis. The mtFAS process is required for mitochondrial respiratory chain assembly and function, synthesis of the lipoic acid cofactor indispensable for the function of several mitochondrial enzyme complexes and essential for embryonic development in mice. Mutations in human mtFAS have been reported to lead to neurodegenerative disease. The source of malonyl-CoA for mtFAS in mammals has remained unclear. We report the identification of a conserved vertebrate mitochondrial isoform of ACC1 expressed from an ACACA transcript splicing variant. A specific knockdown (KD) of the corresponding transcript in mouse cells, or CRISPR/Cas9-mediated inactivation of the putative mitochondrial targeting sequence in human cells, leads to decreased lipoylation and mitochondrial fragmentation. Simultaneous KD of ACSF3, encoding a mitochondrial malonyl-CoA synthetase previously implicated in the mtFAS process, resulted in almost complete ablation of protein lipoylation, indicating that these enzymes have a redundant function in mtFAS. The discovery of a mitochondrial isoform of ACC1 required for lipoic acid synthesis has intriguing consequences for our understanding of mitochondrial disorders, metabolic regulation of mitochondrial biogenesis and cancer.