The present study investigated performance changes during three runs (1 min recovery) of repeated military simulation task test (RMST) and its associations with physical performance. Voluntary male soldiers (N = 114) participated in a series of measurements of physical performance. Lower body explosive force production, anaerobic endurance and upper body strength endurance together explained 58% of the variance in the first RSMT (p < 0.001). The same variables explained the variance in the second and third runs of RSMT by 60% and 51%, but explosive force production was replaced with aerobic endurance, assessed by the 3.2 km loaded run (p < 0.001). This study demonstrated that the role of explosive power of the lower body decreased and military specific aerobic endurance increased when occupational performance was assessed under acute fatigue even during a short high-intensity test. These results may benefit tactical strength and conditioning coaches in training optimization for improved occupational performance in military.