In this study, we developed a deep learning-based 3D markerless motion capture system for skate skiing on a treadmill and evaluated its accuracy against marker-based motion capture during G1 and G3 skating techniques. Participants performed roller skiing trials on a skiing treadmill. Trials were recorded with two synchronized video cameras (100 Hz). We then trained a custom model using DeepLabCut, and the skiing movements were analyzed using both DeepLabCut-based markerless motion capture and marker-based motion capture systems. We statistically compared joint centers and joint vector angles between the methods. The results demonstrated a high level of agreement for joint vector angles, with mean differences ranging from −2.47° to 3.69°. For joint center positions and toe placements, mean differences ranged from 24.0 to 40.8 mm. This level of accuracy suggests that our markerless approach could be useful as a skiing coaching tool. The method presents interesting opportunities for capturing and extracting value from large amounts of data without the need for markers attached to the skier and expensive cameras.